Počet záznamů: 1
Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough
- 1.
SYSNO ASEP 0518352 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough Tvůrce(i) Beznosková, Petra (MBU-M) RID
Pavlíková, Zuzana (MBU-M)
Zeman, Jakub (MBU-M) ORCID
Aitken, C.E. (US)
Valášek, Leoš Shivaya (MBU-M) RID, ORCIDZdroj.dok. Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 47, č. 12 (2019), s. 6339-6350Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Frameshifting ; Codon ; Ribosomal frameshifting Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GA18-02014S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000475891900030 EID SCOPUS 85069293930 DOI 10.1093/nar/gkz346 Anotace Stop codon readthrough-the decoding of a stop codon by a near-cognate tRNA-is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli. Owing to the prevalence of premature termination codons in human disease, readthrough has emerged as an attractive therapeutic target. A growing list of various features, for example the +4 nucleotide immediately following the stop codon, modulate readthrough levels, underscoring the need for systematic investigation of readthrough. Here, we identified and described a complete group of yeast tRNAs that induce readthrough in the stop-codon tetranucleotide manner when overexpressed, designated readthrough-inducing tRNAs (rti-tRNAs). These rti-tRNAs are the keystones of YARIS (yeast applied readthrough inducing system), a reporter-based assay enabling simultaneous detection of readthrough levels at all twelve stop-codon tetranucleotides and as a function of the complete set of rti-tRNAs. We demonstrate the utility of YARIS for systematic study of translation readthrough by employing it to interrogate the effects of natural rti-tRNA modifications, as well as various readthrough-inducing drugs (RTIDs). This analysis identified a variety of genetic interactions demonstrating the power of YARIS to characterize existing and identify novel RTIDs. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://academic.oup.com/nar/article/47/12/6339/5487265
Počet záznamů: 1