Počet záznamů: 1
Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation
- 1.
SYSNO ASEP 0510268 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differentiation of neural rosettes from human pluripotent stem cells in vitro is sequentially regulated on a molecular level and accomplished by the mechanism reminiscent of secondary neurulation Tvůrce(i) Fedorová, V. (CZ)
Váňová, T. (CZ)
Elrefae, L. (CZ)
Pospíšil, J. (CZ)
Petrášová, M. (CZ)
Kolajová, V. (CZ)
Hudačová, Z. (CZ)
Baniariová, J. (CZ)
Barak, M. (CZ)
Pešková, L. (CZ)
Bárta, T. (CZ)
Kaucká, M. (CZ)
Killinger, Michael (UZFG-Y)
Večeřa, J. (CZ)
Bernatík, O. (CZ)
Čajánek, L. (CZ)
Hříbková, H. (CZ)
Bohaciaková, D. (CZ)Číslo článku 101563 Zdroj.dok. Stem Cell Research. - : Elsevier - ISSN 1873-5061
Roč. 40, OCT (2019)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova human embryonic stem cells ; induced pluripotent stem cells ; differentiation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000491224500006 EID SCOPUS 85071716380 DOI 10.1016/j.scr.2019.101563 Anotace Development of neural tube has been extensively modeled in vitro using human pluripotent stem cells (hPSCs) that are able to form radially organized cellular structures called neural rosettes. While a great amount of research has been done using neural rosettes, studies have only inadequately addressed how rosettes are formed and what the molecular mechanisms and pathways involved in their formation are. Here we address this question by detailed analysis of the expression of pluripotency and differentiation-associated proteins during the early onset of differentiation of hPSCs towards neural rosettes. Additionally, we show that the BMP signaling is likely contributing to the formation of the complex cluster of neural rosettes and its inhibition leads to the altered expression of PAX6, SOX2 and SOX1 proteins and the rosette morphology. Finally, we provide evidence that the mechanism of neural rosettes formation in vitro is reminiscent of the process of secondary neurulation rather than that of primary neurulation in vivo. Since secondary neurulation is a largely unexplored process, its understanding will ultimately assist the development of methods to prevent caudal neural tube defects in humans. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://www.sciencedirect.com/science/article/pii/S187350611930193X
Počet záznamů: 1