Počet záznamů: 1
Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response
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SYSNO ASEP 0509034 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response Tvůrce(i) Coufal, Štěpán (MBU-M) ORCID, RID
Galanová, Natálie (MBU-M) ORCID
Bajer, Lukáš (MBU-M)
Gajdárová, Zuzana (MBU-M)
Schierová, Dagmar (MBU-M)
Jirásková Zákostelská, Zuzana (MBU-M) ORCID
Kostovčíková, Klára (MBU-M) ORCID
Jacková, Zuzana (MBU-M)
Stehlíková, Zuzana (MBU-M)
Drastich, P. (CZ)
Tlaskalová-Hogenová, Helena (MBU-M) RID, ORCID
Kverka, Miloslav (MBU-M) RID, ORCIDČíslo článku 719 Zdroj.dok. Cells. - : MDPI
Roč. 8, č. 7 (2019)Poč.str. 20 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova inflammatory bowel disease ; biomarkers ; gut barrier Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NV15-28064A GA MZd - Ministerstvo zdravotnictví NV18-09-00493 GA MZd - Ministerstvo zdravotnictví NV18-05-00394 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000478902000087 DOI 10.3390/cells8070719 Anotace Crohn's disease (CD), ulcerative colitis (UC) and inflammatory bowel disease (IBD) associated with primary sclerosing cholangitis (PSC-IBD), share three major pathogenetic mechanisms of inflammatory bowel disease (IBD)-gut dysbiosis, gut barrier failure and immune system dysregulation. While clinical differences among them are well known, the underlying mechanisms are less explored. To gain an insight into the IBD pathogenesis and to find a specific biomarker pattern for each of them, we used protein array, ELISA and flow cytometry to analyze serum biomarkers and specific anti-microbial B and T cell responses to the gut commensals. We found that decrease in matrix metalloproteinase (MMP)-9 and increase in MMP-14 are the strongest factors discriminating IBD patients from healthy subjects and that PSC-IBD patients have higher levels of Mannan-binding lectin, tissue inhibitor of metalloproteinases 1 (TIMP-1), CD14 and osteoprotegerin than patients with UC. Moreover, we found that low transforming growth factor-P1 (TGF-(31) is associated with disease relapse and low osteoprotegerin with anti-tumor necrosis factor-alpha (TNF-alpha) therapy. Patients with CD have significantly decreased antibody and increased T cell response mainly to genera Eubacterium, Faecalibacterium and Bacteroides. These results stress the importance of the gut barrier function and immune response to commensal bacteria and point at the specific differences in pathogenesis of PSC-IBD, UC and CD. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/2073-4409/8/7/719
Počet záznamů: 1