Počet záznamů: 1
Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin
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SYSNO ASEP 0508305 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin Tvůrce(i) Čížková, Mária (MBU-M) ORCID
Slavková, Monika (MBU-M)
Vítová, Milada (MBU-M) RID, ORCID
Zachleder, Vilém (MBU-M) ORCID
Bišová, Kateřina (MBU-M) RIDČíslo článku 735 Zdroj.dok. Cells. - : MDPI
Roč. 8, č. 7 (2019)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova DNA damage ; double-stranded break ; zeocin Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA15-09231S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000478902000118 DOI 10.3390/cells8070735 Anotace DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter cells. Here, we tested the effect of the antibiotic zeocin, inducing double stranded DNA breaks, on the cell cycle of synchronized cultures of the green alga Chlamydomonas reinhardtii. After zeocin application, DNA replication partially occurred but nuclear and cellular divisions were completely blocked. Application of zeocin combined with caffeine, known to alleviate DNA checkpoints, decreased cell viability significantly. This was probably caused by a partial overcoming of the cell cycle progression block in such cells, leading to aberrant cell divisions. The cell cycle block was accompanied by high steady state levels of mitotic cyclin-dependent kinase activity. The data indicate that DNA damage response in C. reinhardtii is connected to the cell cycle block, accompanied by increased and stabilized mitotic cyclin-dependent kinase activity. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://www.mdpi.com/2073-4409/8/7/735
Počet záznamů: 1