Počet záznamů: 1
Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching
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SYSNO ASEP 0507905 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching Tvůrce(i) Chane, A. (FR)
Barbey, C. (FR)
Robert, M. (FR)
Merieau, A. (FR)
Konto-Ghiorghi, Y. (FR)
Beury-Cirou, A. (FR)
Feuilloley, M. (FR)
Pátek, Miroslav (MBU-M) RID, ORCID
Gobert, V. (FR)
Latour, X. (FR)Zdroj.dok. Molecular Plant-Microbe Interactions. - : American Phytopathological Society - ISSN 0894-0282
Roč. 32, č. 7 (2019), s. 802-812Poč.str. 11 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova green fluorescent protein ; lactone-mediated communication ; n-acylhomoserine lactones Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000473639600003 EID SCOPUS 85066430113 DOI 10.1094/MPMI-11-18-0314-R Anotace Confocal laser-scanning microscopy was chosen to observe the colonization and damage caused by the soft rot Pectobacterium atrosepticum and the protection mediated by the biocontrol agent Rhodococcus erythropolis. We developed dual-color reporter strains suited for monitoring quorum-sensing and quorum-quenching activities leading to maceration or biocontrol, respectively. A constitutively expressed cyan or red fluorescent protein served as a cell tag for plant colonization, while an inducible expression reporter system based on the green fluorescent protein gene enabled the simultaneous recording of signaling molecule production, detection, or degradation. The dual-colored pathogen and biocontrol strains were used to coinoculate potato tubers. At cellular quorum, images revealed a strong pectobacterial quorum-sensing activity, especially at the plant cell walls, as well as a concomitant rhodococcal quorum-quenching response, at both the single-cell and microcolony levels. The generated biosensors appear to be promising and complementary tools useful for molecular and cellular studies of bacterial communication and interference. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-18-0314-R
Počet záznamů: 1