Počet záznamů: 1
Changes in activity of metabolic and regulatory pathways during germination of S-coelicolor
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SYSNO ASEP 0506585 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Changes in activity of metabolic and regulatory pathways during germination of S-coelicolor Tvůrce(i) Bobek, Jan (MBU-M)
Straková, Eva (MBU-M)
Ziková, Alice (MBU-M)
Vohradský, Jiří (MBU-M) RID, ORCIDČíslo článku 1173 Zdroj.dok. BMC Genomics. - : BioMed Central - ISSN 1471-2164
Roč. 15, DEC 23 (2014)Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Germination ; Streptomyces ; Microarrays Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP GAP302/11/0229 GA ČR - Grantová agentura ČR GAP302/10/0468 GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000347731600001 EID SCOPUS 84928235774 DOI 10.1186/1471-2164-15-1173 Anotace Background: Bacterial spore germination is a developmental process during which all required metabolic pathways are restored to transfer cells from their dormant state into vegetative growth. Streptomyces are soil dwelling filamentous bacteria with complex life cycle, studied mostly for they ability to synthesize secondary metabolites including antibiotics.
Results: Here, we present a systematic approach that analyzes gene expression data obtained from 13 time points taken over 5.5 h of Streptomyces germination. Genes whose expression was significantly enhanced/diminished during the time-course were identified, and classified to metabolic and regulatory pathways. The classification into metabolic pathways revealed timing of the activation of specific pathways during the course of germination. The analysis also identified remarkable changes in the expression of specific sigma factors over the course of germination. Based on our knowledge of the targets of these factors, we speculate on their possible roles during germination. Among the factors whose expression was enhanced during the initial part of germination, SigE is though to manage cell wall reconstruction, SigR controls protein re-aggregation, and others (SigH, SigB, SigI, SigJ) control osmotic and oxidative stress responses.
Conclusions: From the results, we conclude that most of the metabolic pathway mRNAs required for the initial phases of germination were synthesized during the sporulation process and stably conserved in the spore. After rehydration in growth medium, the stored mRNAs are being degraded and resynthesized during first hour. From the analysis of sigma factors we conclude that conditions favoring germination evoke stress-like cell responses.Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-1173
Počet záznamů: 1