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6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems
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SYSNO ASEP 0506449 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název 6,7-dimethoxy-coumarin as a probe of hydration dynamics in biologically relevant systems Tvůrce(i) Ghose, Avisek (MBU-M)
Amaro, M. (CZ)
Kovaříček, P. (CZ)
Hof, M. (CZ)
Sýkora, J. (CZ)Číslo článku 025005 Zdroj.dok. Methods and Applications in Fluorescence. - : Institute of Physics Publishing - ISSN 2050-6120
Roč. 6, č. 2 (2018)Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova time dependent fluorescence shift ; 6,7-dimethoxy-coumarin ; hydration Obor OECD Microbiology Způsob publikování Omezený přístup Institucionální podpora MBU-M - RVO:61388971 UT WOS 000425031200001 EID SCOPUS 85048126917 DOI 10.1088/2050-6120/aaaa05 Anotace Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6,7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6,7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6,7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2020 Elektronická adresa https://iopscience.iop.org/article/10.1088/2050-6120/aaaa05
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