Počet záznamů: 1
Expression of selected mitochondrial genes during in vitro maturation of bovine oocytes related to their meiotic competence
- 1.
SYSNO ASEP 0506153 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Expression of selected mitochondrial genes during in vitro maturation of bovine oocytes related to their meiotic competence Tvůrce(i) Němcová, Lucie (UZFG-Y) RID
Hulínská, P. (CZ)
Ješeta, M. (CZ)
Kempisty, B. (PL)
Kaňka, Jiří (UZFG-Y) RID, ORCID
Machatková, M. (CZ)Zdroj.dok. Theriogenelogy. - : Elsevier - ISSN 0093-691X
Roč. 133, JUL 15 (2019), s. 104-112Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova bovine ; oocyte ; maturation Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology CEP QJ1510138 GA MZe - Ministerstvo zemědělství Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000471207900013 EID SCOPUS 85065492262 DOI 10.1016/j.theriogenology.2019.05.001 Anotace The main goal of this study was to characterize the expression patterns of genes which play a role in mitochondrial DNA biogenesis and metabolism during the maturation of bovine oocytes with different meiotic competence and health. Meiotically more and less competent oocytes were obtained separately either from medium (MF) or small (SF) follicles and categorized according to oocyte morphology into healthy and light-atretic. The four oocyte categories were matured and collected after 0, 3, 7, 16 and 24 h of maturation. Either total RNA or poly(A) RNA were extracted from oocytes and the expression of selected mitochondrial translational factors (TFAM, TFBIM, and TFB2M), MATER, and Luciferase as external standard was assessed using a real-time RT-PCR. The level of TFAM, TFBIM and MATER poly(A) RNA transcripts significantly decreased during maturation in both healthy and light-atretic MF and SF oocytes. On the other hand, the level of TFB2M poly(A) increased during maturation in healthy and lightatretic SF oocytes, in contrast to MF oocytes. The abundance of TFAM total RNA was significantly higher after maturation than that before maturation in all oocyte categories. However, no differences in TFB1M and TFB2M total RNA were found in any oocyte categories. It can be concluded that the gene expression patterns differ in maturing bovine oocytes in dependence on their meiotic competence and health. The TFAM and TFBIM poly(A) RNAs are actively deadenylated at different meiotic stages but TFB2M poly(A) RNA remains elevated in light-atretic less competent oocytes until the completion of meiosis. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=7093757609
Počet záznamů: 1