Počet záznamů: 1
In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
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SYSNO ASEP 0506137 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time Tvůrce(i) Hroncová, Z. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Hakl, J. (CZ)
Titěra, D. (CZ)
Havlík, J. (CZ)Číslo článku 110 Zdroj.dok. BMC Microbiology. - : BioMed Central - ISSN 1471-2180
Roč. 19, MAY 24 (2019)Poč.str. 8 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova actinobacteris ; bacteroidetes ; black pupae Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000468899800001 EID SCOPUS 85066396372 DOI 10.1186/s12866-019-1490-y Anotace BackgroundKnowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae.ResultsIndividual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%), however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P<0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P<0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates).ConclusionsAlthough sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1490-y
Počet záznamů: 1