Počet záznamů: 1
Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish
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SYSNO ASEP 0506134 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish Tvůrce(i) Oralová, Veronika (UZFG-Y) RID, ORCID
Rosa, J. T. (BE)
Soenens, M. (BE)
Bek, J. W. (BE)
Willaert, A. (BE)
Witten, P. E. (BE)
Huysseune, A. (BE)Číslo článku UNSP bio042374 Zdroj.dok. Biology Open. - : Company of Biologists - ISSN 2046-6390
Roč. 8, č. 5 (2019)Poč.str. 8 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova zebrafish ; GMA ; GFP ; fluorophores ; immunofluorescence Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000470125600020 EID SCOPUS 85066868667 DOI 10.1242/bio.042374 Anotace Zebrafish is now widely used in biomedical research as a model for human diseases, but the relevance of the model depends on a rigorous analysis of the phenotypes obtained. Many zebrafish disease models, experimental techniques and manipulations take advantage of fluorescent reporter molecules. However, phenotypic analysis often does not go beyond establishing overall distribution patterns of the fluorophore in whole-mount embryos or using vibratome or paraffin sections with poor preservation of tissue architecture and limited resolution. Obtaining high-resolution data of fluorescent signals at the cellular level from internal structures mostly depends on the availability of expensive imaging technology. Here, we propose a new and easily applicable protocol for embedding and sectioning of zebrafish embryos using in-house prepared glycol methacrylate (GMA) plastic that is suited for preservation of fluorescent signals (including photoactivatable fluorophores) without the need for antibodies. Four main approaches are described, all involving imaging fluorescent signals on semithin (3 mu m or less) sections. These include sectioning transgenic animals, whole-mount immunostained embryos, cell tracking, as well as on section enzyme histochemistry. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://bio.biologists.org/content/8/5/bio042374
Počet záznamů: 1