Počet záznamů: 1

Metabolomic Study of Obesity and Its Treatment with Palmitoylated Prolactin-Releasing Peptide Analog in Spontaneously Hypertensive and Normotensive Rats

SYSNO ASEP	0504540
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Metabolomic Study of Obesity and Its Treatment with Palmitoylated Prolactin-Releasing Peptide Analog in Spontaneously Hypertensive and Normotensive Rats
Tvůrce(i)	Čermáková, Martina (MBU-M)_ORCID Pelantová, Helena (MBU-M)_{ORCID, RID} Neprašová, Barbora (FGU-C) Šedivá, Blanka (MBU-M)_ORCID Maletínská, L. (CZ) Kuneš, Jaroslav (FGU-C)_{RID, ORCID} Tomášová, Petra (MBU-M) Železná, B. (CZ) Kuzma, Marek (MBU-M)_{ORCID, RID}
Zdroj.dok.	Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893 Roč. 18, č. 4 (2019), s. 1735-1750
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	NMR ; metabolomics ; obesity
Vědní obor RIV	EE - Mikrobiologie, virologie
Obor OECD	Microbiology
Vědní obor RIV – spolupráce	Fyziologický ústav - Endokrinologie, diabetologie, metabolizmus, výživa
CEP	LO1509 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	GA18-10591S GA ČR - Grantová agentura ČR
Způsob publikování	Omezený přístup
Institucionální podpora	MBU-M - RVO:61388971 ; FGU-C - RVO:67985823
UT WOS	000464068900024
EID SCOPUS	85063953402
DOI	10.1021/acs.jproteome.8b00964
Anotace	In this study, the combination of metabolomics and standard biochemical and biometric parameters was used to describe the metabolic effects of diet-induced obesity and its treatment with the novel antiobesity compound palm(11)-PrRP31 (palmitoylated prolactin-releasing peptide) in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). The results showed that SHR on a high-fat (HF) diet were normoglycemic with obesity and hypertension, while WKY on the HF diet were normotensive and obese with prediabetes. NMR-based metabolomics revealed mainly several microbial cometabolites altered by the HF diet, particularly in urine. The HF diet induced similar changes in both models. However, two groups of genotype-specific metabolites were defined: metabolites specific to the genotype at baseline (e.g., 1-methylnicotinamide, phenylacetylglycine, taurine, methylamine) and metabolites reacting specifically to the HF diet in individual genotypes (2-oxoglutarate, dimethylamine, N-butyrylglycine, p-cresyl sulfate). The palm(11)-PrRP31 lowered body weight and improved biochemical and biometric parameters in both strains, and it improved glucose tolerance in WKY rats on the HF diet. In urine, the therapy induced significant decrease of formate and 1-methylnicotinamide in SHR and alanine, allantoin, dimethylamine, and N-butyrylglycine in WKY. Altogether, our study confirms the effectiveness of palm(11)-PrRP31 for antiobesity treatment.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2020
Elektronická adresa	https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.8b00964

Počet záznamů: 1