Počet záznamů: 1

microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles

SYSNO ASEP	0504365
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	microRNA expression profile in porcine oocytes with different developmental competence derived from large or small follicles
Tvůrce(i)	Gad, Ahmed (UZFG-Y)_ORCID Němcová, Lucie (UZFG-Y)_RID Murín, Matěj (UZFG-Y)_ORCID Kaňka, Jiří (UZFG-Y)_{RID, ORCID} Laurinčík, Jozef (UZFG-Y)_ORCID Benc, M. (SK) Pendovski, L. (MK) Procházka, Radek (UZFG-Y)_{RID, ORCID}
Zdroj.dok.	Molecular Reproduction and Development - ISSN 1040-452X Roč. 86, č. 4 (2019), s. 426-439
Poč.str.	14 s.
Forma vydání	Tištěná - P
Jazyk dok.	eng - angličtina
Země vyd.	US - Spojené státy americké
Klíč. slova	follicular size ; miRNA ; oocyte
Vědní obor RIV	EB - Genetika a molekulární biologie
Obor OECD	Developmental biology
CEP	EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	UZFG-Y - RVO:67985904
UT WOS	000465087200008
EID SCOPUS	85061478251
DOI	10.1002/mrd.23121
Anotace	Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that are essential for oocyte-specific processes throughout the development. The objective of this study was to identify the expression profile of miRNAs in porcine oocytes derived from follicles of different sizes using RNA deep sequencing. Oocytes were aspirated from large (LO, 3-6 mm) or small (SO, 1.5-1.9 mm) follicles and tested for developmental competence and chromatin configurations. Small RNA libraries were constructed from both groups and then sequenced in an Illumina NextSeq. 500. Oocytes from the LO group exhibited higher developmental competence and different chromatin configuration compared with oocytes from the SO group. In total, 167 and 162 known miRNAs were detected in the LO and SO groups, respectively. MiR-205, miR-16, miR-148a-3p, and miR-125b were among the top 10 highly expressed miRNAs in both groups. Eight miRNAs were differentially expressed (DE) between both groups. Target gene prediction and pathway analysis revealed 46 pathways that were enriched with miRNA-target genes. The oocyte meiosis pathway and signaling pathways including FoxO, PI3K-Akt, and cAMP were predictably targeted by DE miRNAs. These results give more insights into the potential role of miRNAs in regulating the oocyte development.
Pracoviště	Ústav živočišné fyziologie a genetiky
Kontakt	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Rok sběru	2020
Elektronická adresa	https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=61644394257

Počet záznamů: 1