Počet záznamů: 1
The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid
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SYSNO ASEP 0503711 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid Tvůrce(i) Pocherniaieva, K. (CZ)
Güralp, H. (CZ)
Saito, T. (CZ)
Pšenička, M. (CZ)
Tichopád, T. (CZ)
Janko, Karel (UZFG-Y) RID, ORCID
Kašpar, V. (CZ)Zdroj.dok. Turkish Journal of Fisheries and Aquatic Sciences. - : Central Fisheries Research Institute - ISSN 1303-2712
Roč. 19, č. 2 (2019), s. 167-174Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. TR - Turecko Klíč. slova cell cycle ; DAPI ; synchronization Vědní obor RIV EG - Zoologie Obor OECD Zoology CEP GA17-09807S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000577400900009 EID SCOPUS 85053340233 DOI 10.4194/1303-2712-v19_2_09 Anotace Detailed knowledge of early embryo development is a prerequisite for implementation of reproductive biotechnologies. We described the timing of mid-blastula transition in the sturgeon Acipenser ruthenus and hybrid of A. ruthenus and Acipenser gueldenstaedtii. To identify mitotic changes during cell division, the nuclei of embryo blastomeres were sampled every 30 min from 4 to 25 h post-fertilization and stained with 5 mg/ml 4'-6-diaminido-2phenylindole. We observed embryos of hybrids to lose synchrony after the eighth cell division, one cycle earlier than in purebred sterlet embryos. To reveal the timing of zygotic gene activation, developing embryos were dechorionated and injected with 10, 50, 100, 250, or 500 mu g/ml a-Amanitin. Termination of development in embryos was recorded throughout the period from the 1000 cell stage until gastrulation, showing the process of transition from maternal to zygotic gene transcription in sterlet to be of extended duration and not directly linked to mid-blastula transition. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa http://trjfas.org/abstract.php?id=1328
Počet záznamů: 1