Počet záznamů: 1
Fragment of the aspartyl-tRNA synthetase applicable as a shared classification and phylogenetic marker in particular representatives of the order Lactobacillales
- 1.
SYSNO ASEP 0503657 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Fragment of the aspartyl-tRNA synthetase applicable as a shared classification and phylogenetic marker in particular representatives of the order Lactobacillales Tvůrce(i) Mekadim, Chahrazed (UZFG-Y) ORCID
Killer, Jiří (UZFG-Y) RID, ORCID
Pechar, R. (CZ)
Mrázek, Jakub (UZFG-Y) RID, ORCIDZdroj.dok. Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 64, č. 1 (2019), s. 113-120Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Lactobacillales ; Lactid-acid bacteria Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy NV16-27449A GA MZd - Ministerstvo zdravotnictví Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 000455916000014 EID SCOPUS 85051864456 DOI 10.1007/s12223-018-0638-8 Anotace The order Lactobacillales represents a morphologically, metabolically, and physiologically diverse group of bacteria. Lactic acid bacteria represent the core of this phylogenetic group. They are a part of epiphytic microflora, fermented dairy, meat, fruit and vegetable products, and the digestive tract of humans and animals. Despite the fact that these bacteria form a phenotypically and genotypically heterogeneous group, their phylogenetic relationship enables to propose a common genetic marker usable in classification, typing, and phylogeny. By creation of consensus sequence based on available genomic sequences of some representatives of order Lactobacillales, a specific primer-pair binding variable region of aspS gene (length of 615 nts) encoding the aspartyl-tRNA synthetase was designed. This gene has not yet been used in classification and phylogeny of the order Lactobacillales, although it meets the requirements of molecular markers (distribution and single copy in bacterial genomes, functional constancy and genetic stability, sequence variability among taxonomic units, irreplaceable role in proteosynthesis). Primers were applied on 54 type and wild Lactobacillales strains. Obtained sequences allowed to provide alignments for purpose of phylogenetic tree reconstructions that uncovered particular phylogenetic clusters of vagococci/enterococci, obligately homofermentative and heterofermentative lactobacilli. Although a relatively short fragment of the aspS gene (approximately 33% of the complete gene sequence) was evaluated, much higher sequence variability (61.8% of pairwise identity) among strains examined compared with 16S rRNA gene (90.7%, length of 1318nt) provides a relatively simple and effective tool for classification and typing of selected representatives of the order Lactobacillales. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2020 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=53700017215
Počet záznamů: 1