Počet záznamů: 1
Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals
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SYSNO ASEP 0499508 Druh ASEP C - Konferenční příspěvek (mezinárodní konf.) Zařazení RIV D - Článek ve sborníku Název Nanofiber dressing consisting of antisense rna-functionalized nanodiamonds for therapy of non-healing wounds in diabetic individuals Tvůrce(i) Neuhoferová, Eva (MBU-M) ORCID
Petráková, V. (CZ)
Vocetková, K. (CZ)
Kindermann, Marek (MBU-M)
Křivohlavá, Romana (MBU-M) RID
Benson, Veronika (MBU-M) RID, ORCIDZdroj.dok. CONFERENCE PROCEEDINGS NANOCON 2017. - Brno : Tnager Ltd., 2018 - ISBN 9788087294819 Rozsah stran s. 467-472 Poč.str. 6 s. Forma vydání Tištěná - P Akce 9th International Conference on Nanomaterials - Research and Application (NANOCON) Datum konání 18.10.2017 - 20.10.2017 Místo konání Brno Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova Electrospun nanofibers ; fluorescent nanodiamonds ; RNA interference Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NV15-33094A GA MZd - Ministerstvo zdravotnictví Institucionální podpora MBU-M - RVO:61388971 UT WOS 000452823300076 EID SCOPUS 85052321107 Anotace Non-healing wounds are serious complication in diabetic patients and represent an attractive challenge for development of suitable carrier system possessing constant and localized release of therapeutic biomolecule into the wound without any undesired side effects. Given the fact that these non-healing wounds are result of impaired balance in metalloproteinases synthesized by immune cells residing the wounds, gene therapy offering knock down of such enzymes is of great interest.
Here we challenged a development of functional and biocompatible wound dressing enabling controlled release of trackable carrier loaded with therapeutic siRNA. Our dressing consists of scaffold from degradable polymer nanofibers enriched with fluorescent nanodiamond particles (FND). We have previously shown the nanodiamond particles are great carriers for antisense RNAs. Their advantages represent high biocompatibility, stable luminescence giving us the possibility to track the carrier system in the wound, and effective release of antisense RNA in the wound. Embedding of nanodiamond-siRNA systems into nanofiber scaffold enables continuous release of siRNA and maintaining the stable siRNA concentration in the wound site resulting in a promotion of wound healing.
We developed FND-siRNA complexes specific to MMP-9 that efficiently inhibit the expression of target MMP-9 mRNA. The complexes were embedded into core/shell nanofibers from PVA and PCL, visualized by confocal microscopy, and characterized by electron microscopy. Real-time PCR was used to assess the silencing effect of siRNA that has been delivered to target murine fibroblasts by FND released from nanofiber dressing. Nanofiber system with embedded FNDs was applied on wounds in diabetic animal models to evaluate its suitability regarding short and long term toxicity, efficacy, and handling in vivo.
Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
Počet záznamů: 1