Počet záznamů: 1
Freezing processes in cryoprotective solutions of ME.sub.2./sub.SO, trehalose and antifreeze protein APAFP752 and their impact on chromatin condition of cryopreserved cells
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SYSNO ASEP 0494040 Druh ASEP A - Abstrakt Zařazení RIV O - Ostatní Název Freezing processes in cryoprotective solutions of ME2SO, trehalose and antifreeze protein APAFP752 and their impact on chromatin condition of cryopreserved cells Tvůrce(i) Golan, Martin (FZU-D) RID
Sedláková, Silvia (FZU-D) RID, ORCID
Mičová, Júlia (FZU-D)
Šebera, Jakub (FZU-D) RID
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Richter, J. (CZ)
Řeha, David (MBU-M) ORCID
Falk, Martin (BFU-R) RID, ORCID
Falková, Iva (BFU-R) ORCID
Bačíková, Alena (BFU-R)
Pagáčová, Eva (BFU-R) ORCID
Varga, K. (US)Zdroj.dok. Cryobiology. - : Elsevier - ISSN 0011-2240
Roč. 71, č. 3 (2015), s. 561-561Poč.str. 1 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova cryoprotective agents ; freezing ; cryopreservation Vědní obor RIV BO - Biofyzika Obor OECD Biophysics Institucionální podpora FZU-D - RVO:68378271 ; MBU-M - RVO:61388971 ; BFU-R - RVO:68081707 DOI 10.1016/j.cryobiol.2015.10.101 Anotace In our work, we studied the behaviour of aqueous solutions of cryoprotective agents (Me2SO, trehalose, antifreeze protein ApAFP752) upon freezing/thawing. We characterized the course of phase change of all the s ranging from 20°C to -170°C using differential scanning calorimetry (shape and position of freezing/thawing curves) and Raman spectroscopy (molecular and intermolecular structure). We correlate our experimental results with molecular dynamics‘ modelling of interactions of solution constituents. We also show that antifreeze protein ApAFP752 has thermal hysteresis activity and is able to increase viability of Xenopus oocytes when microinjected into them prior to cryopreservation. Pracoviště Fyzikální ústav Kontakt Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Rok sběru 2019
Počet záznamů: 1