Počet záznamů: 1

Activation/Inactivation Role of Ionic Liquids on Formate Dehydrogenase from Pseudomonas sp 101 and Its Mutated Thermostable Form

SYSNO ASEP	0493016
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Activation/Inactivation Role of Ionic Liquids on Formate Dehydrogenase from Pseudomonas sp 101 and Its Mutated Thermostable Form
Tvůrce(i)	D'Oronzo, E. (IT) Secundo, F. (IT) Minofar, Babak (MBU-M)_{RID, ORCID} Kulik, Natalia (MBU-M)_ORCID Pometun, A.A. (RU) Tishkov, V.I. (RU)
Zdroj.dok.	ChemCatChem. - : Wiley - ISSN 1867-3880 Roč. 10, č. 15 (2018), s. 3247-3259
Poč.str.	13 s.
Jazyk dok.	eng - angličtina
Země vyd.	DE - Německo
Klíč. slova	enzymes ; fluorescence spectroscopy ; ionic liquids
Vědní obor RIV	CE - Biochemie
Obor OECD	Biochemistry and molecular biology
Institucionální podpora	MBU-M - RVO:61388971
UT WOS	000442208200009
EID SCOPUS	85047490820
DOI	10.1002/cctc.201800145
Anotace	Ionic liquids (ILs) are used in numerous research areas including biocatalysis. The effect of ILs/water mixture on the activity of wild type and a more thermally and chemically stable mutant (SM4) of a specific formate dehydrogenases (PseFDH, EC1.2.1.2) were studied experimentally and by molecular dynamics (MD) simulations. The ILs investigated were [Mmim][Me2PO4], [Bmim][Br], [Bmim][CH3SO3], [Bmim][BF4], [Bmim][AcO], and it was found that low concentrations (optimally 2.5%) of some ILs increased (up to 42%) the activity of the SM4 FDH but not of the WT FDH. Using intrinsic fluorescence to calculate Stern-Volmer constants and thermodynamic parameters, we have studied protein conformational changes caused by ILs for both enzymes. Kinetic analyses allowed us to shed light on the mechanism of activation by 2.5% [Bmim][BF4] on the mutant enzyme. MD simulation provided evidences of a molecular basis of different enzyme activities in ILs that well correlated with the experimental data.
Pracoviště	Mikrobiologický ústav
Kontakt	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Rok sběru	2019

Počet záznamů: 1