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Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast
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SYSNO ASEP 0490196 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast Tvůrce(i) Bednařík, A. (CZ)
Kuta, J. (CZ)
Vu, Dai Long (MBU-M) ORCID
Ranglová, Karolína (MBU-M) ORCID
Hrouzek, Pavel (MBU-M) ORCID
Kanický, V. (CZ)
Preisler, J. (CZ)Zdroj.dok. Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1533, JAN 19 (2018), s. 199-207Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Diode laser thermal vaporization (DLTV) ; Thin-layer chromatography (TLC) ; Liquid chromatography (LC) Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP LO1416 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora MBU-M - RVO:61388971 UT WOS 000424187500022 EID SCOPUS 85039045431 DOI 10.1016/j.chroma.2017.12.017 Anotace In this work we present a simple and cost-effective approach for the determination of selenium species in algae and yeast biomass, based on a combination of thin-layer chromatography (TLC) with diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). Extraction of freeze-dried biomass was performed in 4M methanesulphonic acid and the selenium species were vaporized from cellulose TLC plates employing a continuous-wave infrared diode laser with power up to 4W using a simple laboratory-built apparatus. Selenomethionine and selenocysteine were quantified with limits of detection 3 mu g L-1 in a Se-enriched microalgae Chlorella vulgaris and yeast certified reference material SELM-1. Results delivered by TLC-DLTV ICP MS were consistent with those obtained by a routine coupling of high-performance liquid chromatography (HPLC) to ICP MS. In addition, the TLC approach is capable of analyzing extract containing even undiluted crude hydrolysates that could damage HPLC columns. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2019
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