Počet záznamů: 1  

MLL2 conveys transcription-independent H3K4 trimethylation in oocytes

    1.
    SYSNO ASEP0488581
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevMLL2 conveys transcription-independent H3K4 trimethylation in oocytes
    Tvůrce(i) Hanna, C. W. (GB)
    Taudt, A. (NL)
    Huang, J. (GB)
    Gahurová, Lenka (UZFG-Y) ORCID
    Kranz, A. (DE)
    Andrews, S. (GB)
    Dean, W. (GB)
    Francis Stewart, A. (DE)
    Colomé-Tatché, M. (NL)
    Kelsey, G. (GB)
    Zdroj.dok.Nature Structural & Molecular Biology. - : Nature Publishing Group - ISSN 1545-9993
    Roč. 25, č. 1 (2018), s. 73-82
    Poč.str.10 s.
    Forma vydáníTištěná - P
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovaH3K4 trimethylation
    Vědní obor RIVEB - Genetika a molekulární biologie
    Obor OECDReproductive biology (medical aspects to be 3)
    Institucionální podporaUZFG-Y - RVO:67985904
    UT WOS000423547700011
    DOI10.1038/s41594-017-0013-5
    AnotaceHistone 3 K4 trimethylation (depositing H3K4me3 marks) is typically associated with active promoters yet paradoxically occurs at untranscribed domains. Research to delineate the mechanisms of targeting H3K4 methyltransferases is ongoing. The oocyte provides an attractive system to investigate these mechanisms, because extensive H3K4me3 acquisition occurs in nondividing cells. We developed low-input chromatin immunoprecipitation to interrogate H3K4me3, H3K27ac and H3K27me3 marks throughout oogenesis. In nongrowing oocytes, H3K4me3 was restricted to active promoters, but as oogenesis progressed, H3K4me3 accumulated in a transcription-independent manner and was targeted to intergenic regions, putative enhancers and silent H3K27me3-marked promoters. Ablation of the H3K4 methyltransferase gene Mll2 resulted in loss of transcription-independent H3K4 trimethylation but had limited effects on transcription-coupled H3K4 trimethylation or gene expression. Deletion of Dnmt3a and Dnmt3b showed that DNA methylation protects regions from acquiring H3K4me3. Our findings reveal two independent mechanisms of targeting H3K4me3 to genomic elements, with MLL2 recruited to unmethylated CpG-rich regions independently of transcription.
    PracovištěÚstav živočišné fyziologie a genetiky
    KontaktJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Rok sběru2019
Počet záznamů: 1  

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