Počet záznamů: 1
Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth
- 1.
SYSNO ASEP 0469864 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth Tvůrce(i) Bloch, D. (FR)
Pleskot, Roman (UEB-Q) RID, ORCID
Pejchar, Přemysl (UEB-Q) RID, ORCID
Potocký, Martin (UEB-Q) RID, ORCID
Trpkošová, Pavlína (UEB-Q)
Cwiklik, Lukasz (UFCH-W) RID, ORCID
Vukašinović, Nemanja (UEB-Q) ORCID
Sternberg, H. (IL)
Yalovsky, S. (IL)
Žárský, Viktor (UEB-Q) RID, ORCIDCelkový počet autorů 10 Zdroj.dok. Plant Physiology. - : Oxford University Press - ISSN 0032-0889
Roč. 172, č. 2 (2016), s. 980-1002Poč.str. 23 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova polar cell-growth ; arabidopsis-thaliana ; plasma-membrane ; vesicle trafficking ; affects endocytosis ; subunit sec3 ; force-field ; tip growth ; complex ; tobacco Vědní obor RIV EB - Genetika a molekulární biologie Vědní obor RIV – spolupráce Ústav fyzikální chemie J.Heyrovského - Fyzikální chemie a teoretická chemie CEP GA13-19073S GA ČR - Grantová agentura ČR Institucionální podpora UEB-Q - RVO:61389030 ; UFCH-W - RVO:61388955 UT WOS 000391147700028 DOI https://doi.org/10.1104/pp.16.00690 Anotace Polarized exocytosis is critical for pollen tube growth, but its localization and function are still under debate. The exocyst vesicletethering complex functions in polarized exocytosis. Here, we show that a sec3a exocyst subunit null mutant cannot be transmitted through the male gametophyte due to a defect in pollen tube growth. The green fluorescent protein (GFP)-SEC3a fusion protein is functional and accumulates at or proximal to the pollen tube tip plasma membrane. Partial complementation of sec3a resulted in the development of pollen with multiple tips, indicating that SEC3 is required to determine the site of pollen germination pore formation. Time-lapse imaging demonstrated that SEC3a and SEC8 were highly dynamic and that SEC3a localization on the apical plasma membrane predicts the direction of growth. At the tip, polar SEC3a domains coincided with cell wall deposition. Labeling of GFP-SEC3a-expressing pollen with the endocytic marker FM4-64 revealed the presence of subdomains on the apical membrane characterized by extensive exocytosis. In steady-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin homology-like domain (SEC3a-N)-dependent subapical membrane localization. In agreement, SEC3a-N interacted with phosphoinositides in vitro and colocalized with a phosphatidylinositol 4,5-bisphosphate (PIP2) marker in pollen tubes. Correspondingly, molecular dynamics simulations indicated that SEC3a-N associates with the membrane by interacting with PIP2. However, the interaction with PIP2 is not required for polar localization and the function of SEC3a in Arabidopsis (Arabidopsis thaliana). Taken together, our findings indicate that SEC3a is a critical determinant of polar exocytosis during tip growth and suggest differential regulation of the exocytotic machinery depending on pollen tube growth modes. Pracoviště Ústav experimentální botaniky Kontakt David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Rok sběru 2017
Počet záznamů: 1