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Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development
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SYSNO ASEP 0482431 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evolutionary history of callose synthases in terrestrial plants with emphasis on proteins involved in male gametophyte development Author(s) Záveská Drábková, Lenka (UEB-Q) ORCID
Honys, David (UEB-Q) RID, ORCIDNumber of authors 2 Article number e0187331 Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 12, č. 11 (2017)Number of pages 24 s. Language eng - English Country US - United States Keywords exine pattern-formation ; pollen wall pattern ; arabidopsis-thaliana ; nicotiana-tabacum ; gene-expression ; transcriptome analysis ; male-fertility ; udp-glucose ; family ; diversification Subject RIV EB - Genetics ; Molecular Biology OECD category Plant sciences, botany R&D Projects GA15-16050S GA ČR - Czech Science Foundation (CSF) GA17-23183S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000414997800010 DOI 10.1371/journal.pone.0187331 Annotation Callose is a plant-specific polysaccharide (beta-1,3-glucan) playing an important role in angio-sperms in many developmental processes and responses to biotic and abiotic stresses. Callose is synthesised at the plasma membrane of plant cells by callose synthase (CalS) and, among others, represents the main polysaccharide in the callose wall surrounding the tetrads of developing microspores and in the growing pollen tube wall. CalS proteins involvement in spore development is a plesiomorphic feature of terrestrial plants, but very little is known about their evolutionary origin and relationships amongst the members of this protein family. We performed thorough comparative analyses of callose synthase family proteins from major plant lineages to determine their evolutionary history across the plant kingdom. A total of 1211 candidate CalS sequences were identified and compared amongst diverse taxonomic groups of plants, from bryophytes to angiosperms. Phylogenetic analyses identified six main clades of CalS proteins and suggested duplications during the evolution of specialised functions. Twelve family members had previously been identified in Arabidopsis thaliana. We focused on five CalS subfamilies directly linked to pollen function and found that proteins expressed in pollen evolved twice. CalS9/10 and CalS11/12 formed well-defined clades, whereas pollen-specific CalS5 was found within subfamilies that mostly did not express in mature pollen vegetative cell, although were found in sperm cells. Expression of five out of seven mature pollen-expressed CalS genes was affected by mutations in bzip transcription factors. Only three subfamilies, CalS5, CalS10, and CalS11, however, formed monophyletic, mostly conserved clades. The pairs CalS9/CalS10, CalS11/CalS12 and CalS3 may have diverged after angiosperms diversified from lycophytes and bryophytes. Our analysis of fully sequenced plant proteins identified new evolutionary lineages of callose synthase subfamilies and has established a basis for understanding their functional evolution in terrestrial plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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