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Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization

SYSNO ASEP	0461616
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization
Author(s)	Hafidh, Said (UEB-Q)_{RID, ORCID} Potěšil, D. (CZ) Fíla, Jan (UEB-Q)_{RID, ORCID} Čapková, Věra (UEB-Q)_RID Zdráhal, Z. (CZ) Honys, David (UEB-Q)_{RID, ORCID}
Article number	81
Source Title	Genome Biology - ISSN 1465-6906 Roč. 17, MAY 3 (2016)
Number of pages	29 s.
Language	eng - English
Country	US - United States
Keywords	Protein secretion ; Pollen tube guidance ; Cell-cell signaling
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA15-22720S GA ČR - Czech Science Foundation (CSF)
	GA14-32292S GA ČR - Czech Science Foundation (CSF)
	GA15-16050S GA ČR - Czech Science Foundation (CSF)
	LD14109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2015043 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0068 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LQ1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UEB-Q - RVO:61389030
UT WOS	000375962000001
DOI	10.1186/s13059-016-0928-x
Annotation	We performed genome-wide quantitative liquid chromatography-tandem mass spectrometry analysis of a pistil-stimulated pollen tube secretome and identified 801 pollen tube-secreted proteins. Interestingly, in silico analysis reveals that the pollen tube secretome is dominated by proteins that are secreted unconventionally, representing 57 % of the total secretome. In support, we show that an unconventionally secreted protein, translationally controlled tumor protein, is secreted to the apoplast. Remarkably, we discovered that this protein could be secreted by infiltrating through the initial phases of the conventional secretory pathway and could reach the apoplast via exosomes, as demonstrated by co-localization with Oleisin1 exosome marker. We demonstrate that translationally controlled tumor protein-knockdown Arabidopsis thaliana plants produce pollen tubes that navigate poorly to the target ovule and that the mutant allele is poorly transmitted through the male. Further, we show that regulators of the endoplasmic reticulum-trans-Golgi network protein secretory pathway control secretion of Nicotiana tabacum Pollen tube-secreted cysteine-rich protein 2 and Lorelei-like GPI-anchor protein 3 and that a regulator of endoplasmic reticulum-trans-Golgi protein translocation is essential for pollen tube growth, pollen tube guidance and ovule-targeting competence.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2017

Number of the records: 1