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cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase

SYSNO ASEP	0456689
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase
Author(s)	Dokládal, Ladislav (BFU-R) Honys, David (UEB-Q)_{RID, ORCID} Rana, Rajiv (UEB-Q) Lee, L.-Y. (US) Gelvin, S.B. (US) Sýkorová, Eva (BFU-R)_{RID, ORCID}
Number of authors	6
Source Title	Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X Roč. 6, NOV2015 (2015)
Number of pages	11 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	telomerase ; nuclear poly(A)-binding protein ; telobox
Subject RIV	BO - Biophysics
Subject RIV - cooperation	Institute of Experimental Botany - Botanics
R&D Projects	GA13-06943S GA ČR - Czech Science Foundation (CSF)
	ED1.1.00/02.0068 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707 ; UEB-Q - RVO:61389030
UT WOS	000366252400001
DOI	10.3389/fpls.2015.00985
Annotation	Telomerase-reverse transcriptase (TERT) plays an essential catalytic role in maintaining telomeres. However, in animal systems telomerase plays additional non-telomeric functional roles. We previously screened an Arabidopsis cDNA library for proteins that interact with the C-terminal extension (GTE) TERT domain and identified a nuclearlocalized protein that contains an RNA recognition motif (RRM). This RRM-protein forms homodimers in both plants and yeast. Mutation of the gene encoding the RRM-protein had no detectable effect on plant growth and development, nor did it affect telomerase activity or telomere length in vivo, suggesting a non-telomeric role for TERT/RRM-protein complexes. The gene encoding the RRM-protein is highly expressed in leaf and reproductive tissues. We further screened an Arabiclopsis cDNA library for proteins that interact with the RRM-protein and identified five interactors. These proteins are involved in numerous non-telomere-associated cellular activities.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2016

Number of the records: 1