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Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.

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    SYSNO ASEP0456197
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleOxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.
    Author(s) Pelclová, D. (CZ)
    Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
    Kačer, P. (CZ)
    Fenclová, Z. (CZ)
    Vlčková, Š. (CZ)
    Syslová, K. (CZ)
    Navrátil, Tomáš (UFCH-W) RID, ORCID
    Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
    Zíková, Naděžda (UCHP-M) RID, ORCID, SAI
    Barošová, H. (CZ)
    Turci, F. (IT)
    Komarc, M. (CZ)
    Pelcl, T. (CZ)
    Běláček, J. (CZ)
    Kukutschová, J. (CZ)
    Zakharov, S. (CZ)
    Source TitleJournal of Breath Research - ISSN 1752-7155
    Roč. 10, č. 1 (2016), s. 016004
    Number of pages11 s.
    Languageeng - English
    CountryGB - United Kingdom
    Keywordsnanoparticles ; exhaled breath condensate ; oxidative stress ; urine ; occupational exposure ; Fe2O3 ; Fe3O4
    Subject RIVDN - Health Impact of the Environment Quality
    OBOR OECDPublic and environmental health
    Subject RIV - cooperationJ. Heyrovsky Institute of Physical Chemistry - Physical ; Theoretical Chemistry
    Institutional supportUCHP-M - RVO:67985858 ; UFCH-W - RVO:61388955
    UT WOS000375749700006
    EID SCOPUS84983215770
    AnnotationMarkers of oxidative stress and inflammation were analysed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43±7 years) exposed to iron oxide aerosol for an average of 10±4 years and 14 controls (mean age 39±4 years) by liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083mg m(-3) (IQR 0.063-0.133mg m(-3)) and the median particle concentration was 66800 particles cm(-3) (IQR 16900-86900 particles cm(-3)). In addition, more than 80% of particles were smaller than 100nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p<0.001), and C11 (p<0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC.
    WorkplaceInstitute of Chemical Process Fundamentals
    ContactEva Jirsová,, Tel.: 220 390 227
    Year of Publishing2016
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