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Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.

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    0456197 - ÚCHP 2016 RIV GB eng J - Journal Article
    Pelclová, D. - Ždímal, Vladimír - Kačer, P. - Fenclová, Z. - Vlčková, Š. - Syslová, K. - Navrátil, Tomáš - Schwarz, Jaroslav - Zíková, Naděžda - Barošová, H. - Turci, F. - Komarc, M. - Pelcl, T. - Běláček, J. - Kukutschová, J. - Zakharov, S.
    Oxidative Stress Markers Are Elevated in Exhaled Breath Condensate of Workers Exposed to Nanoparticles during Iron Oxide Pigment Production.
    Journal of Breath Research. Roč. 10, č. 1 (2016), s. 016004. ISSN 1752-7155
    Institutional support: RVO:67985858 ; RVO:61388955
    Keywords : nanoparticles * exhaled breath condensate * oxidative stress * urine * occupational exposure * Fe2O3 * Fe3O4
    Subject RIV: DN - Health Impact of the Environment Quality; CF - Physical ; Theoretical Chemistry (UFCH-W)
    OBOR OECD: Public and environmental health; Physical chemistry (UFCH-W)
    Impact factor: 4.318, year: 2016

    Markers of oxidative stress and inflammation were analysed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43±7 years) exposed to iron oxide aerosol for an average of 10±4 years and 14 controls (mean age 39±4 years) by liquid chromatography-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction. Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083mg m(-3) (IQR 0.063-0.133mg m(-3)) and the median particle concentration was 66800 particles cm(-3) (IQR 16900-86900 particles cm(-3)). In addition, more than 80% of particles were smaller than 100nm in diameter. Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2alpha (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analysed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p<0.001), and C11 (p<0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC.

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