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Transcriptome profiling of male gametophyte development in Nicotiana tabacum
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SYSNO ASEP 0454988 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptome profiling of male gametophyte development in Nicotiana tabacum Author(s) Bokvaj, Pavel (UEB-Q)
Hafidh, Said (UEB-Q) RID, ORCID
Honys, David (UEB-Q) RID, ORCIDSource Title GENOMICS DATA - ISSN 2213-5960
Roč. 3, MAR (2015), s. 106-111Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords Pollen development transcriptome ; Tobacco ; Reproduction Subject RIV EB - Genetics ; Molecular Biology R&D Projects GAP501/11/1462 GA ČR - Czech Science Foundation (CSF) GAP305/12/2611 GA ČR - Czech Science Foundation (CSF) LD14109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA13-06943S GA ČR - Czech Science Foundation (CSF) LD13049 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000362674200026 DOI 10.1016/j.gdata.2014.12.002 Annotation Pollen, an extremely reduced bicellular or tricellular male reproductive structure of flowering plants, serves as a model for numerous studies covering wide range of developmental and physiological processes. The pollen development represents a fragile and vital phase of plant ontogenesis and pollen was among the first singular plant tissues thoroughly characterized at the transcriptomic level (Honys and Twell [5]). Arabidopsis pollen developmental transcriptome has been published over a decade ago (Honys and Twell, 2004) and transcriptomes of developing pollen of other species have followed (Rice, Deveshwar et al. [2]; Triticeae, Tranet al. [11]; upland cotton, Ma et al. [8]). However, the transcriptomic data describing the development of tobacco pollen, a bicellular model for cell biology studies, have been missing. Here we provide the transcriptomic data covering three stages (Tupy et al., 1983) of wild type tobacco (Nicotiana tabacum, cv. Samsun) pollen development: uninucleate microspores (UNM, stage 1), early bicellular pollen (eBCP, stage 3) and late bicellular pollen (lBCP, stage 5) as a supplement to the mature pollen (MP), 4 h-pollen tube (PT4), 24 h-pollen tubes (PT24), leaf (LF) and root (RT) transcriptomic data presented in our previous studies (Hafidh et al., 2012a; Hafidh et al., 2012b). We characterized these transcriptomes to refine the knowledge base of male gametophyte-enriched genes as well as genes expressed preferentially at the individual stages of pollen development. Alongside updating the list of tissue-specific genes, we have investigated differentially expressed genes with respect to early expressed genes. Pollen tube growth and competition of pollen tubes in female pistil can be viewed as a race of the fittest. Accordingly, there is an apparent evolutionary trend among higher plants to store significant material reserves and nutrients during pollen maturation. This supply ensures that after pollen germination, the pollen tube utilizes its resource predominantly for its rapid elongation in the female pistil. Previous transcriptomic data from Arabidopsis showed massive expression of genes encoding proteins forming both ribosomal subunits that were accumulated in developing pollen, whereas their expression was not detectable in growing pollen tubes (Honys and Twell, 2004). We observed a similar phenomenon in less advanced bicellular tobacco pollen. Here, we describe in detail how we obtained and analyzed validated microarray dataset deposited in Gene Expression Omnibus (GSE62349). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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