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Clonal analysis of hematopoietic progenitor cells in the zebrafish
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SYSNO ASEP 0365457 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Clonal analysis of hematopoietic progenitor cells in the zebrafish Author(s) Stachura, D.L. (US)
Svoboda, Ondřej (UMG-J)
Lau, R.P. (US)
Balla, K.M. (US)
Zon, L.I. (US)
Bartůněk, Petr (UMG-J) RID
Traver, D. (US)Source Title Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 118, č. 5 (2011), s. 1274-1282Number of pages 9 s. Language eng - English Country US - United States Keywords zebrafish ; in vitro culture ; hematopoiesis Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA310/08/0878 GA ČR - Czech Science Foundation (CSF) GAP305/10/0953 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000293510000019 DOI 10.1182/blood-2011-01-331199 Annotation Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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