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Clonal analysis of hematopoietic progenitor cells in the zebrafish

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    0365457 - ÚMG 2012 RIV US eng J - Journal Article
    Stachura, D.L. - Svoboda, Ondřej - Lau, R.P. - Balla, K.M. - Zon, L.I. - Bartůněk, Petr - Traver, D.
    Clonal analysis of hematopoietic progenitor cells in the zebrafish.
    Blood. Roč. 118, č. 5 (2011), s. 1274-1282. ISSN 0006-4971
    R&D Projects: GA ČR GA310/08/0878; GA ČR GAP305/10/0953
    Institutional research plan: CEZ:AV0Z50520514
    Keywords : zebrafish * in vitro culture * hematopoiesis
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 9.898, year: 2011

    Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis.
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