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Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

SYSNO ASEP	0546003
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata
Author(s)	Šolínová, Veronika (UOCHB-X)_{RID, ORCID} Sázelová, Petra (UOCHB-X)_{RID, ORCID} Mášová, Alice (UOCHB-X) Jiráček, Jiří (UOCHB-X)_{RID, ORCID} Kašička, Václav (UOCHB-X)_{RID, ORCID}
Article number	5636
Source Title	Molecules. - : MDPI Roč. 26, č. 18 (2021)
Number of pages	13 s.
Language	eng - English
Country	CH - Switzerland
Keywords	antimicrobial peptides ; beta-alanyl-tyrosine ; capillary zone electrophoresis ; free-flow zone electrophoresis ; peptide analysis ; peptide purification
OECD category	Analytical chemistry
R&D Projects	GA20-03899S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UOCHB-X - RVO:61388963
UT WOS	000701860300001
EID SCOPUS	85115318448
DOI	10.3390/molecules26185636
Annotation	The problem of a growing resistance of bacteria and other microorganisms to conventional antibiotics gave rise to a search for new potent antimicrobial agents. Insect antimicrobial peptides (AMPs) seem to be promising novel potential anti-infective therapeutics. The dipeptide β-alanyl-tyrosine (β-Ala-Tyr) is one of the endogenous insect toxins exhibiting antibacterial activity against both Gram-negative and Gram-positive bacteria. Prior to testing its other antimicrobial activities, it has to be prepared in a pure form. In this study, we have developed a capillary zone electrophoresis (CZE) method for analysis of β-Ala-Tyr isolated from the extract of the hemolymph of larvae of the fleshfly Neobellieria bullata by reversed-phase high-performance liquid chromatography (RP-HPLC). Based on our previously described correlation between CZE and free-flow zone electrophoresis (FFZE), analytical CZE separation of β-Ala-Tyr and its admixtures have been converted into preparative purification of β-Ala-Tyr by FFZE with preparative capacity of 45.5 mg per hour. The high purity degree of the β-Ala-Tyr obtained by FFZE fractionation was confirmed by its subsequent CZE analysis.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2022
Electronic address	https://doi.org/10.3390/molecules26185636

Number of the records: 1