Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata

0546003 - ÚOCHB 2022 RIV CH eng J - Journal Article
Šolínová, Veronika - Sázelová, Petra - Mášová, Alice - Jiráček, Jiří - Kašička, Václav
Application of Capillary and Free-Flow Zone Electrophoresis for Analysis and Purification of Antimicrobial β-Alanyl-Tyrosine from Hemolymph of Fleshfly Neobellieria bullata.
Molecules. Roč. 26, č. 18 (2021), č. článku 5636. E-ISSN 1420-3049
R&D Projects: GA ČR(CZ) GA20-03899S; GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : antimicrobial peptides * beta-alanyl-tyrosine * capillary zone electrophoresis * free-flow zone electrophoresis * peptide analysis * peptide purification
OECD category: Analytical chemistry
Impact factor: 4.927, year: 2021
Method of publishing: Open access
https://doi.org/10.3390/molecules26185636

The problem of a growing resistance of bacteria and other microorganisms to conventional antibiotics gave rise to a search for new potent antimicrobial agents. Insect antimicrobial peptides (AMPs) seem to be promising novel potential anti-infective therapeutics. The dipeptide β-alanyl-tyrosine (β-Ala-Tyr) is one of the endogenous insect toxins exhibiting antibacterial activity against both Gram-negative and Gram-positive bacteria. Prior to testing its other antimicrobial activities, it has to be prepared in a pure form. In this study, we have developed a capillary zone electrophoresis (CZE) method for analysis of β-Ala-Tyr isolated from the extract of the hemolymph of larvae of the fleshfly Neobellieria bullata by reversed-phase high-performance liquid chromatography (RP-HPLC). Based on our previously described correlation between CZE and free-flow zone electrophoresis (FFZE), analytical CZE separation of β-Ala-Tyr and its admixtures have been converted into preparative purification of β-Ala-Tyr by FFZE with preparative capacity of 45.5 mg per hour. The high purity degree of the β-Ala-Tyr obtained by FFZE fractionation was confirmed by its subsequent CZE analysis.
Permanent Link: http://hdl.handle.net/11104/0322596