Number of the records: 1

Characterization of alba family expression and localization in arabidopsis thaliana generative organs

SYSNO ASEP	0545878
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Characterization of alba family expression and localization in arabidopsis thaliana generative organs
Author(s)	Náprstková, Alena (UEB-Q)_ORCID Malínská, Kateřina (UEB-Q)_{RID, ORCID} Záveská Drábková, Lenka (UEB-Q)_ORCID Billey, E. (FR) Náprstková, Dagmar (UEB-Q) Sýkorová, Eva (BFU-R)_{RID, ORCID} Bousquet-Antonelli, C. (FR) Honys, David (UEB-Q)_{RID, ORCID}
Number of authors	8
Article number	1652
Source Title	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 4 (2021)
Number of pages	23 s.
Language	eng - English
Country	CH - Switzerland
Keywords	alba ; Arabidopsis thaliana ; Confocal microscopy ; Expression analysis ; Flowering ; Heat stress ; pabp3 ; Pollen development ; Protein localization
OECD category	Plant sciences, botany
R&D Projects	GA18-07027S GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707
UT WOS	000623761000001
EID SCOPUS	85100487109
DOI	10.3390/ijms22041652
Annotation	ALBA DNA/RNA-binding proteins form an ancient family, which in eukaryotes diversi-fied into two Rpp25-like and Rpp20-like subfamilies. In most studied model organisms, their function remains unclear, but they are usually associated with RNA metabolism, mRNA translatability and stress response. In plants, the enriched number of ALBA family members remains poorly understood. Here, we studied ALBA dynamics during reproductive development in Ara-bidopsis at the levels of gene expression and protein localization, both under standard conditions and following heat stress. In generative tissues, ALBA proteins showed the strongest signal in mature pollen where they localized predominantly in cytoplasmic foci, particularly in regions surrounding the vegetative nucleus and sperm cells. Finally, we demonstrated the involvement of two Rpp25-like subfamily members ALBA4 and ALBA6 in RNA metabolism in mature pollen supported by their co-localization with poly(A)-binding protein 3 (PABP3). Collectively, we demonstrated the engagement of ALBA proteins in male reproductive development and the heat stress response, highlighting the involvement of ALBA4 and ALBA6 in RNA metabolism, storage and/or translational control in pollen upon heat stress. Such dynamic re-localization of ALBA proteins in a controlled, developmentally and environmentally regulated manner, likely reflects not only their redundancy but also their possible functional diversification in plants.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2022
Electronic address	http://doi.org/10.3390/ijms22041652

Number of the records: 1