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Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations

SYSNO ASEP	0543809
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations
Author(s)	Konášová, Renáta (UOCHB-X)_ORCID Butnariu, Maria (UOCHB-X)_ORCID Šolínová, Veronika (UOCHB-X)_{RID, ORCID} Kašička, Václav (UOCHB-X)_{RID, ORCID} Koval, Dušan (UOCHB-X)_{RID, ORCID}
Article number	338789
Source Title	Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670 Roč. 1178, Sep 15 (2021)
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	electroosmotic flow ; capillary electrophoresis ; mass spectrometry ; permanent polymeric coating ; sorption suppression
OECD category	Analytical chemistry
R&D Projects	GA20-03899S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Limited access
Institutional support	UOCHB-X - RVO:61388963
UT WOS	000672564300008
EID SCOPUS	85109129026
DOI	10.1016/j.aca.2021.338789
Annotation	Electroosmotic flow (EOF) plays a pivotal role in optimization of capillary electrophoresis (CE) separations of (bio)molecules and (bio)particles. EOF velocity is directly related to analysis time, peak resolution and separation efficiency. Here, we report a concept of charged polymer coatings of the inner fused silica capillary wall, which allows anodic EOF with mobility ranging from 0 to ∼(30–40) × 10−9 m2V−1s−1. The capillary wall is modified by covalently bound cationic copolymer poly(acrylamide-co-(3-acrylamidopropyl)trimethylammonium chloride) (PAMAPTAC) containing variable ratio of the charged monomer in the 0–60 mol. % interval. The EOF mobility showed minor variability with composition of background electrolyte (BGE) and pH in the 2–10 interval. The coatings were evaluated by CE-UV and nanospray CE-MS in the counter-EOF arrangement for a series of basic drug molecules in acetic acid based acidic BGE. Tunable EOF velocity was demonstrated as a useful tool for optimization of peak resolution, separation efficiency and migration time of analytes. Electrostatic repulsion of positively charged capillary surface was shown as beneficial for suppression of analyte adsorption, notably for hydrophobic cationic analytes.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2022
Electronic address	https://doi.org/10.1016/j.aca.2021.338789

Number of the records: 1