Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations

0543809 - ÚOCHB 2022 RIV NL eng J - Journal Article
Konášová, Renáta - Butnariu, Maria - Šolínová, Veronika - Kašička, Václav - Koval, Dušan
Covalent cationic copolymer coatings allowing tunable electroosmotic flow for optimization of capillary electrophoretic separations.
Analytica Chimica Acta. Roč. 1178, Sep 15 (2021), č. článku 338789. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA ČR(CZ) GA20-03899S
Institutional support: RVO:61388963
Keywords : electroosmotic flow * capillary electrophoresis * mass spectrometry * permanent polymeric coating * sorption suppression
OECD category: Analytical chemistry
Impact factor: 6.911, year: 2021
Method of publishing: Limited access
https://doi.org/10.1016/j.aca.2021.338789

Electroosmotic flow (EOF) plays a pivotal role in optimization of capillary electrophoresis (CE) separations of (bio)molecules and (bio)particles. EOF velocity is directly related to analysis time, peak resolution and separation efficiency. Here, we report a concept of charged polymer coatings of the inner fused silica capillary wall, which allows anodic EOF with mobility ranging from 0 to ∼(30–40) × 10−9 m2V−1s−1. The capillary wall is modified by covalently bound cationic copolymer poly(acrylamide-co-(3-acrylamidopropyl)trimethylammonium chloride) (PAMAPTAC) containing variable ratio of the charged monomer in the 0–60 mol. % interval. The EOF mobility showed minor variability with composition of background electrolyte (BGE) and pH in the 2–10 interval. The coatings were evaluated by CE-UV and nanospray CE-MS in the counter-EOF arrangement for a series of basic drug molecules in acetic acid based acidic BGE. Tunable EOF velocity was demonstrated as a useful tool for optimization of peak resolution, separation efficiency and migration time of analytes. Electrostatic repulsion of positively charged capillary surface was shown as beneficial for suppression of analyte adsorption, notably for hydrophobic cationic analytes.
Permanent Link: http://hdl.handle.net/11104/0320927