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Analysis of peptide mixtures for proteomics research using LC–ESI-MS with a simple microgradient device
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SYSNO ASEP 0447214 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Analysis of peptide mixtures for proteomics research using LC–ESI-MS with a simple microgradient device Author(s) Lenobel, R. (CZ)
Řehulková, H. (CZ)
Šebela, M. (CZ)
Franc, V. (CZ)
Kahle, Vladislav (UIACH-O) RID
Moravcová, Dana (UIACH-O) RID, ORCID
Řehulka, P. (CZ)Number of authors 7 Source Title Lc Gc North America - ISSN 1527-5949
Roč. 33, č. 6 (2015), s. 420-428Number of pages 7 s. Publication form Print - P Language eng - English Country US - United States Keywords LC-ESI-MS ; proteomics ; peptide Subject RIV CB - Analytical Chemistry, Separation R&D Projects VG20112015021 GA MV - Ministry of Interior (MV) Institutional support UIACH-O - RVO:68081715 UT WOS 000356844200005 Annotation This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography and electrospray ionization tandem mass spectrometry. The system was successfully tested for a complex peptide mixture originating from the digestion of the whole-cell lysate from Francisella tularensis subsp. Schu S4 and it represents a cheap alternative for the separation of both simple and complex peptide mixtures prior to MS analysis. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2016 Electronic address http://hdl.handle.net/11104/0249120
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