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Analysis of peptide mixtures for proteomics research using LC–ESI-MS with a simple microgradient device

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    0447214 - ÚIACH 2016 RIV US eng J - Journal Article
    Lenobel, R. - Řehulková, H. - Šebela, M. - Franc, V. - Kahle, Vladislav - Moravcová, Dana - Řehulka, P.
    Analysis of peptide mixtures for proteomics research using LC–ESI-MS with a simple microgradient device.
    Lc Gc North America. Roč. 33, č. 6 (2015), s. 420-428. ISSN 1527-5949. E-ISSN 1939-1889
    R&D Projects: GA MV VG20112015021
    Institutional support: RVO:68081715
    Keywords : LC-ESI-MS * proteomics * peptide
    Subject RIV: CB - Analytical Chemistry, Separation
    Impact factor: 0.519, year: 2014

    This work presents an on-line combination of a simple microgradient device for reversed-phase liquid chromatography and electrospray ionization tandem mass spectrometry. The system was successfully tested for a complex peptide mixture originating from the digestion of the whole-cell lysate from Francisella tularensis subsp. Schu S4 and it represents a cheap alternative for the separation of both simple and complex peptide mixtures prior to MS analysis.

    Permanent Link: http://hdl.handle.net/11104/0249120

     
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