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Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure
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SYSNO ASEP 0424731 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure Author(s) Falk, Martin (BFU-R) RID, ORCID
Lukášová, Emilie (BFU-R) RID, ORCID
Štefančíková, Lenka (BFU-R)
Baranová, E. (CZ)
Falková, Iva (BFU-R) ORCID
Ježková, L. (CZ)
Davídková, Marie (UJF-V) RID, ORCID, SAI
Bačíková, Alena (BFU-R)
Vachelová, Jana (UJF-V) RID, SAI
Jelínek Michaelidesová, Anna (UJF-V) RID, ORCID, SAI
Kozubek, Stanislav (BFU-R) RIDNumber of authors 11 Source Title Applied Radiation and Isotopes. - : Elsevier - ISSN 0969-8043
Roč. 83, Jan (2014), s. 177-185Number of pages 9 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords DNA double strand break (DSB) repair ; Immature and terminally differentiated granulocytes ; gamma H2AX/53BP1 repair foci Subject RIV BO - Biophysics Subject RIV - cooperation Nuclear Physics Institute - Biophysics R&D Projects LD12039 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BFU-R - RVO:68081707 ; UJF-V - RVO:61389005 UT WOS 000329539900020 EID SCOPUS 84889102715 DOI 10.1016/j.apradiso.2013.01.029 Annotation Cell differentiation is associated with extensive gene silencing, heterochromatinization and potentially decreasing need for repairing DNA double-strand breaks (DSBs). Differentiation stages of blood cells thus represent an excellent model to study DSB induction, repair and misrepair in the context of changing higher-order chromatin structure. We show that immature granulocytes form gamma H2AX and 53BP1 foci, contrary to the mature cells; however, these foci colocalize only rarely and DSB repair is inefficient. Moreover, specific chromatin structure of granulocytes probably influences DSB induction. (C) 2013 Elsevier Ltd. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2014
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