Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure

0424731 - BFÚ 2014 RIV GB eng J - Journal Article
Falk, Martin - Lukášová, Emilie - Štefančíková, Lenka - Baranová, E. - Falková, Iva - Ježková, L. - Davídková, Marie - Bačíková, Alena - Vachelová, Jana - Jelínek Michaelidesová, Anna - Kozubek, Stanislav
Heterochromatinization associated with cell differentiation as a model to study DNA double strand break induction and repair in the context of higher-order chromatin structure.
Applied Radiation and Isotopes. Roč. 83, Jan (2014), s. 177-185. ISSN 0969-8043
R&D Projects: GA MŠMT(CZ) LD12039
Institutional support: RVO:68081707 ; RVO:61389005
Keywords : DNA double strand break (DSB) repair * Immature and terminally differentiated granulocytes * gamma H2AX/53BP1 repair foci
Subject RIV: BO - Biophysics; BO - Biophysics (UJF-V)
Impact factor: 1.231, year: 2014

Cell differentiation is associated with extensive gene silencing, heterochromatinization and potentially decreasing need for repairing DNA double-strand breaks (DSBs). Differentiation stages of blood cells thus represent an excellent model to study DSB induction, repair and misrepair in the context of changing higher-order chromatin structure. We show that immature granulocytes form gamma H2AX and 53BP1 foci, contrary to the mature cells; however, these foci colocalize only rarely and DSB repair is inefficient. Moreover, specific chromatin structure of granulocytes probably influences DSB induction. (C) 2013 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0230757