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Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
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SYSNO ASEP 0422636 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer Author(s) Lišková, Marcela (UIACH-O)
Klepárník, Karel (UIACH-O) RID, ORCID
Pazdera, P. (CZ)
Foret, František (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Chemické Listy. Roč. 107, Issue s3.. - Praha : Česká společnost chemická, 2013 - ISSN 0009-2770 Pages s. 315-317 Number of pages 3 s. Publication form Online - E Action CECE Junior 2013 Event date 12.11.2013-13.11.2013 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords caspase-3 ; Förster resonance energy transfer ; apoptotic cells Subject RIV CB - Analytical Chemistry, Separation R&D Projects EE2.3.20.0182 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP301/11/2055 GA ČR - Czech Science Foundation (CSF) GBP206/12/G014 GA ČR - Czech Science Foundation (CSF) GAP206/11/2377 GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000328730800013 Annotation Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2014
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