Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer

0422636 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Lišková, Marcela - Klepárník, Karel - Pazdera, P. - Foret, František
Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 315-317. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA MŠk(CZ) EE2.3.20.0182; GA ČR(CZ) GAP301/11/2055; GA ČR(CZ) GBP206/12/G014; GA ČR GAP206/11/2377
Institutional support: RVO:68081715
Keywords : caspase-3 * Förster resonance energy transfer * apoptotic cells
Subject RIV: CB - Analytical Chemistry, Separation

Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
Permanent Link: http://hdl.handle.net/11104/0228836