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Enrichment techniques employed in phosphoproteomics

SYSNO ASEP	0366394
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Enrichment techniques employed in phosphoproteomics
Author(s)	Fíla, Jan (UEB-Q)_{RID, ORCID} Honys, David (UEB-Q)_{RID, ORCID}
Source Title	Amino Acids. - : Springer - ISSN 0939-4451 Roč. 43, č. 3 (2012), s. 1025-1047
Number of pages	23 s.
Language	eng - English
Country	AT - Austria
Keywords	Phosphoproteomics ; Enrichment ; IMAC
Subject RIV	ED - Physiology
R&D Projects	GAP501/11/1462 GA ČR - Czech Science Foundation (CSF)
	GA522/09/0858 GA ČR - Czech Science Foundation (CSF)
	GA525/09/0994 GA ČR - Czech Science Foundation (CSF)
	OC08011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000307536100003
DOI	10.1007/s00726-011-1111-z
Annotation	Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2013

Number of the records: 1