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Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation
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SYSNO ASEP 0352888 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation Author(s) Hovorka, Ondřej (MBU-M)
Šubr, Vladimír (UMCH-V) RID, ORCID
Větvička, David (MBU-M)
Kovář, Lubomír (MBU-M)
Strohalm, Jiří (UMCH-V)
Strohalm, Martin (MBU-M)
Benda, Aleš (UFCH-W) RID, ORCID
Hof, Martin (UFCH-W) RID, ORCID
Ulbrich, Karel (UMCH-V) RID
Říhová, Blanka (MBU-M) RIDSource Title European Journal of Pharmaceutics and Biopharmaceutics. - : Elsevier - ISSN 0939-6411
Roč. 76, č. 3 (2010), s. 514-524Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords doxorubicin ; spectral analysis ; fluorescence Subject RIV EC - Immunology R&D Projects IAA400200702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAAX00500803 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) AV0Z40400503 - UFCH-W (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000285279900027 DOI 10.1016/j.ejpb.2010.07.008 Annotation We define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement od doxorubicin fluorescence inside the cells Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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