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Membrane Active Peptides. Methods and Results on Structure and Function

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    SYSNO ASEP0348380
    Document TypeM - Monograph Chapter
    R&D Document TypeMonograph Chapter
    TitlePeptide-Membrane Interactions Studied by Ellipsometry, Laser Scanning Microscopy, and Z-Scan Fluorescence Correlation Spectroscopy
    Author(s) Miszta, Adam (UFCH-W)
    Macháň, Radek (UFCH-W)
    Hermens, W. Th. (NL)
    Hof, Martin (UFCH-W) RID, ORCID
    Source TitleMembrane Active Peptides. Methods and Results on Structure and Function. - La Jolla : International University Line, 2010 / Castanho M. A. R. B. - ISBN 978-0-9720774-5-3
    Pagess. 219-245
    Number of pages27 s.
    Number of pages635
    Languageeng - English
    CountryUS - United States
    Keywordsellipsometry ; laser scanning microscopy ; fluorescence microscopy
    Subject RIVCF - Physical ; Theoretical Chemistry
    R&D ProjectsLC06063 GA MŠk - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z40400503 - UFCH-W (2005-2011)
    DOI10.3890/978-0-9720774-5-3
    AnnotationThe following chapter presents two optical experimental techniques: confocal fluorescence microscopy (CFM) and ellipsometry, and focuses on their applications in studying interactions of antimicrobial peptides with supported phospholipid bilayers (SPBs). The principal properties of SPBs are mentioned together with their use as geometrically well-defined models of biological membranes. The basics of each of techniques are explained and their possibilities and limitations are discussed. Images with diffraction-limited resolution are obtained by CFM showing the spatial distribution of fluorescent molecules in the sample. Selective labeling offers the possibility to visualize location of different molecular species. Furthermore, the signal from each point of the sample can be analyzed separately; the measurement of molecular mobility in individual points of the sample by fluorescence correlation spectroscopy is addressed in more detail.
    WorkplaceJ. Heyrovsky Institute of Physical Chemistry
    ContactMichaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
    Year of Publishing2011
Number of the records: 1