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Membrane Active Peptides. Methods and Results on Structure and Function

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    0348380 - ÚFCH JH 2011 RIV US eng M - Monography Chapter
    Miszta, Adam - Macháň, Radek - Hermens, W. Th. - Hof, Martin
    Peptide-Membrane Interactions Studied by Ellipsometry, Laser Scanning Microscopy, and Z-Scan Fluorescence Correlation Spectroscopy.
    Membrane Active Peptides. Methods and Results on Structure and Function. La Jolla: International University Line, 2010 - (Castanho, M.), s. 219-245. IUL Biotechnology Series. ISBN 978-0-9720774-5-3
    R&D Projects: GA MŠk(CZ) LC06063
    Institutional research plan: CEZ:AV0Z40400503
    Keywords : ellipsometry * laser scanning microscopy * fluorescence microscopy
    Subject RIV: CF - Physical ; Theoretical Chemistry

    The following chapter presents two optical experimental techniques: confocal fluorescence microscopy (CFM) and ellipsometry, and focuses on their applications in studying interactions of antimicrobial peptides with supported phospholipid bilayers (SPBs). The principal properties of SPBs are mentioned together with their use as geometrically well-defined models of biological membranes. The basics of each of techniques are explained and their possibilities and limitations are discussed. Images with diffraction-limited resolution are obtained by CFM showing the spatial distribution of fluorescent molecules in the sample. Selective labeling offers the possibility to visualize location of different molecular species. Furthermore, the signal from each point of the sample can be analyzed separately; the measurement of molecular mobility in individual points of the sample by fluorescence correlation spectroscopy is addressed in more detail.
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