Number of the records: 1
Vacuolin-1-modulated exocytosis and cell resealing in mast cells
- 1.
SYSNO ASEP 0334054 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Vacuolin-1-modulated exocytosis and cell resealing in mast cells Author(s) Shaik, Gouse Mohiddin (UMG-J)
Dráberová, Lubica (UMG-J) RID
Heneberg, Petr (UMG-J)
Dráber, Petr (UMG-J) RIDNumber of authors 4 Source Title Cellular Signalling. - : Elsevier - ISSN 0898-6568
Roč. 21, č. 8 (2009), s. 1337-1345Number of pages 9 s. Language eng - English Country GB - United Kingdom Keywords vacuolin-1 ; exocytosis ; mast cells Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA301/09/1826 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000267099800011 DOI 10.1016/j.cellsig.2009.04.001 Annotation The small chemical vacuolin-1 induces rapid formation of large vacuoles in various cell types. In epithelial cells, vacuolin-1 has been shown to inhibit Ca2+ ionophore-induced exocytosis depending on experimental conditions used but had no effect on repair of damaged membranes. However, it is not known whether vacuolin-1 could inhibit exocytosis induced by immunoreceptor triggering in professional secretory cells and whether there is any correlation between effect of vacuolin-1 on exocytosis and membrane repair in such cells. Here we show that in rat basophilic leukemia (RBL-2H3) cells activated by the high-affinity IgE receptor (FcepsilonRI) triggering vacuolin-1 enhanced exocytosis. Under identical conditions of activation, vacuolin-1 inhibited exocytosis in mouse bone marrow-derived mast cells. Our data indicate that lysosomal exocytosis exhibits different sensitivity to vacuolin-1 depending on the cell type analyzed and mode of activation. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
Number of the records: 1