Vacuolin-1-modulated exocytosis and cell resealing in mast cells

0334054 - ÚMG 2010 RIV GB eng J - Journal Article
Shaik, Gouse Mohiddin - Dráberová, Lubica - Heneberg, Petr - Dráber, Petr
Vacuolin-1-modulated exocytosis and cell resealing in mast cells.
Cellular Signalling. Roč. 21, č. 8 (2009), s. 1337-1345. ISSN 0898-6568. E-ISSN 1873-3913
R&D Projects: GA MŠMT 1M0506; GA MŠMT LC545; GA ČR GA301/09/1826
Institutional research plan: CEZ:AV0Z50520514
Keywords : vacuolin-1 * exocytosis * mast cells
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.094, year: 2009

The small chemical vacuolin-1 induces rapid formation of large vacuoles in various cell types. In epithelial cells, vacuolin-1 has been shown to inhibit Ca2+ ionophore-induced exocytosis depending on experimental conditions used but had no effect on repair of damaged membranes. However, it is not known whether vacuolin-1 could inhibit exocytosis induced by immunoreceptor triggering in professional secretory cells and whether there is any correlation between effect of vacuolin-1 on exocytosis and membrane repair in such cells. Here we show that in rat basophilic leukemia (RBL-2H3) cells activated by the high-affinity IgE receptor (FcepsilonRI) triggering vacuolin-1 enhanced exocytosis. Under identical conditions of activation, vacuolin-1 inhibited exocytosis in mouse bone marrow-derived mast cells. Our data indicate that lysosomal exocytosis exhibits different sensitivity to vacuolin-1 depending on the cell type analyzed and mode of activation.
Permanent Link: http://hdl.handle.net/11104/0178889