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Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization
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SYSNO ASEP 0328268 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytoskeleton-Associated Large RNP Complexes in Tobacco Male Gametophyte (EPPs) Are Associated with Ribosomes and Are Involved in Protein Synthesis, Processing, and Localization Title Velké RNP komplexy asociované s cytoskeletem obsahují ribosomy a podílejí se na syntéze bílkovin a jejich zpracování a lokalizaci Author(s) Honys, David (UEB-Q) RID, ORCID
Reňák, David (UEB-Q) RID
Feciková, Jana (UEB-Q) ORCID
Jedelský, P.L. (CZ)
Nebesářová, Jana (BC-A) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Čapková, Věra (UEB-Q) RIDSource Title Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
Roč. 8, č. 8 (2009), s. 2015-2031Number of pages 17 s. Language eng - English Country US - United States Keywords Nicotiana tabacum ; male gametophyte ; pollen Subject RIV EB - Genetics ; Molecular Biology R&D Projects OC08011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000264928200040 DOI 10.1021/pr8009897 Annotation Here, we report the first structural and proteomic data about large ribonucleoprotein particles (EPPs) in tobacco male gametophyte. These complexes are formed in immature pollen where they contain translationally silent mRNAs. Although massively activated at the early progamic phase, they also serve as a long-term storage of mRNA transported along with the translational machinery to the tip region. Moreover, EPPs were shown to contain ribosomal subunits, rRNAs and a set of mRNAs. Presented results extend our view of EPP complexes from mere RNA storage and transport compartment in particular stages of pollen development to the complex and well-organized machinery devoted to mRNA storage, transport and subsequent controlled activation resulting in protein synthesis, processing and precise localization. Moreover, presented complex role of EPPs in tobacco cytoplasmic mRNA and protein metabolism makes them likely to be active in another plant species too. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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