Number of the records: 1

Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis

SYSNO ASEP	0305774
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis
Title	Derivatizace aminokyselin fluoralkychlormravenčany pro analýzy plynovou chromatografií
Author(s)	Hušek, Petr (BC-A) Šimek, Petr (BC-A)_{RID, ORCID} Hartvich, Petr (BC-A) Zahradníčková, Helena (BC-A)_{RID, ORCID}
Source Title	Journal of Chromatography A. - : Elsevier - ISSN 0021-9673 Roč. 1186, 1/2 (2008), s. 391-400
Number of pages	10 s.
Language	eng - English
Country	NL - Netherlands
Keywords	amino acids ; derivatization ; pentafluoropropyl- and heptafluorobutyl chloroformates
Subject RIV	CB - Analytical Chemistry, Separation
R&D Projects	GA203/04/0192 GA ČR - Czech Science Foundation (CSF)
	GA303/06/1674 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
DOI	10.1016/j.chroma.2007.11.117
Annotation	Novel fluoroalkyl chloroformates with 3 and 4 carbon atoms were investigated to immediate conversion of amino acids into hydrophobic derivatives in water-containing media. Derivatization conditions were extensively studied and optimized sample preparation protocols elaborated. More than 30 amino acids were treated with the particular reagent in isooctane by simply vortexing the reactive organic phase with a slightly basified aqueous medium containing pyridine or 3-picoline as a catalyst. Outstanding separation of nearly all components on 5% phenylmethylsilicone phase in gas chromatographic (GC) analysis with mass spectrometric (MS) or flame ionization detection (FID) required <10 min. Quantitation characteristics involving linearity in range of 0.1 to 100 nmol, MS limit of detection (LOD, 0.03 pmol - 20 pmol) and electron impact (EI) spectra and diagnostic SIM fragment ions of the derivatives are reported. The novel method is simple, robust and rapid, running in aqueous environment.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2008

Number of the records: 1