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Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry

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    0460136 - MBU-M 2017 RIV US eng J - Journal Article
    Pompach, Petr - Kavan, Daniel - Benada, Oldřich - Růžička, V. - Volný, M. - Novák, Petr
    Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry.
    Clinical Chemistry. Roč. 62, č. 1 (2016), s. 270-278. ISSN 0009-9147
    R&D Projects: GA MŠk LO1509; GA MŠk(CZ) EE2.3.30.0003; GA MŠk(CZ) ED1.1.00/02.0109
    Institutional support: RVO:61388971
    Keywords : TREATED METAL-SURFACE * IN-SITU ENRICHMENT * ELECTROSPRAY DEPOSITION
    Subject RIV: CE - Biochemistry
    Impact factor: 8.008, year: 2016

    Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable method for high-throughput clinical applications. Mass spectrometry (MS) can in principle provide fast phenotyping because haptoglobin a 1 and a 2, which define the phenotype, have different molecular masses. Because of the complexity of the serum matrix, an efficient and fast enrichment technique is necessary for an MS-based assay.
    Permanent Link: http://hdl.handle.net/11104/0260273