Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry

0460136 - MBÚ 2017 RIV US eng J - Journal Article
Pompach, Petr - Kavan, Daniel - Benada, Oldřich - Růžička, V. - Volný, M. - Novák, Petr
Planar Functionalized Surfaces for Direct Immunoaffinity Desorption/Ionization Mass Spectrometry.
Clinical Chemistry. Roč. 62, č. 1 (2016), s. 270-278. ISSN 0009-9147. E-ISSN 1530-8561
R&D Projects: GA MŠMT LO1509; GA MŠMT(CZ) EE2.3.30.0003; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : TREATED METAL-SURFACE * IN-SITU ENRICHMENT * ELECTROSPRAY DEPOSITION
Subject RIV: CE - Biochemistry
Impact factor: 8.008, year: 2016

Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable method for high-throughput clinical applications. Mass spectrometry (MS) can in principle provide fast phenotyping because haptoglobin a 1 and a 2, which define the phenotype, have different molecular masses. Because of the complexity of the serum matrix, an efficient and fast enrichment technique is necessary for an MS-based assay.
Permanent Link: http://hdl.handle.net/11104/0260273