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Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis
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SYSNO ASEP 0575767 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis Author(s) Klodová, Božena (UEB-Q) ORCID
Potěšil, D. (CZ)
Steinbachová, Lenka (UEB-Q) ORCID, RID
Michailidis, Christos (UEB-Q) ORCID
Lindner, A.C. (DE)
Hackenberg, D. (GB)
Becker, J. D. (PT)
Zdráhal, Z. (CZ)
Twell, D. (GB)
Honys, David (UEB-Q) RID, ORCIDNumber of authors 10 Source Title Plant Reproduction. - : Springer - ISSN 2194-7953
Roč. 36, č. 3 (2023), s. 213-241Number of pages 29 s. Language eng - English Country DE - Germany Keywords Arabidopsis ; Male gametophyte ; RNA-seq ; Proteome OECD category Plant sciences, botany R&D Projects LTC18034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTAUSA18115 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20028 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LTC20050 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA21-15856S GA ČR - Czech Science Foundation (CSF) GA19-01723S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000871865600001 EID SCOPUS 85161836853 DOI 10.1007/s00497-023-00471-w Annotation Sexual reproduction in angiosperms requires the production and delivery of two male gametes by a three-celled haploid male gametophyte. This demands synchronized gene expression in a short developmental window to ensure double fertilization and seed set. While transcriptomic changes in developing pollen are known for Arabidopsis, no studies have integrated RNA and proteomic data in this model. Further, the role of alternative splicing has not been fully addressed, yet post-transcriptional and post-translational regulation may have a key role in gene expression dynamics during microgametogenesis. We have refined and substantially updated global transcriptomic and proteomic changes in developing pollen for two Arabidopsis accessions. Despite the superiority of RNA-seq over microarray-based platforms, we demonstrate high reproducibility and comparability. We identify thousands of long non-coding RNAs as potential regulators of pollen development, hundreds of changes in alternative splicing and provide insight into mRNA translation rate and storage in developing pollen. Our analysis delivers an integrated perspective of gene expression dynamics in developing Arabidopsis pollen and a foundation for studying the role of alternative splicing in this model. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1007/s00497-023-00471-w
Number of the records: 1